Normal görünüm MARC görünümü ISBD görünümü

Chromosome analysis protocols / edited by John R. Gosden. - xi, 508 pages : illustrations (some color) ; 24 cm. - Methods in molecular biology ; v. 29 . - Methods in molecular biology (Clifton, N.J.) ; v. 29. .

Includes bibliographical references and index.

Chromosome sorting by flow cytometry: production of DNA libraries and gene mapping -- Electrophoretic karyotype analysis: pulsed field gel electrophoresis -- Immunofluorescence techniques applied to mitotic chromosome preparations -- Immunocytochemical techniques applied to meiotic chromosomes -- Immortalized cell lines: their creation and use in gene mapping -- Oligonucleotide primed in situ DNA synthesis (PRINS): an alternative to in situ hybridization for gene mapping and the inversion of genome organization -- Electron microscopic localization of in situ hybrids -- Protocols for chromosome-mediated gene transfer: selection strategies, transgenome analysis, enrichment cloning and mapping -- Construction of chromosome-specific libraries of yeast artificial chromosome recombinants from somatic hybrid cell lines Yeast artificial chromosome recombinants in a global strategy for chromosome mapping: amplification of internal and terminal fragments by PCR and generation of fingerprints -- Chromosome dissection and cloning -- Molecular analysis of chromosome aberrations in hematological malignancies: southern hybridization -- Molecular analysis of chromosome aberrations: in situ hybridization -- Alu- and L1-primed PCR-generated probes for nonisotopic in situ hybridization -- Chromosome substructure investigation: telomeres Mitotic metaphase chromosome preparation from peripheral blood for high resolution -- Chromosome preparation from hematological malignancies -- Meiotic chromosome preparation -- Preparation of chromosomes for scanning electron microscopy -- Immortalized cell lines: chromosome preparation and banding -- Chromosome banding and identification absorption staining -- Chromosome banding and identification: fluorescence -- Chromosome banding: stain combinations for specific regions -- Inhibition of chromosome condensation -- Analysis of chromosomes with restriction endonucleases and DNase hypersensitivity -- Automatic karyotype analysis -- Bivariate chromosome analysis using a commercial flow cytometer /Judith A. Fantes, Daryll K. Green, Andrew Sharkey /John Maule /Peter Jeppesen /Christa Heyting ... [and others] /Veronica Van Heyningen /John R. Gosden, Diane Lawson /Sandya Narayanswami, Barbara A. Hamkalo /David J. Porteous /Benoit Arveiler. /Benoit Arveiler /Steven D.M. Brown, Alysson H. Carey /David L. Saltman, Stephen P. Hunger, Gillian E. Turner /Peter Lichter, Thomas Ried /John R. Gosden, Matthew Breen, Diane Lawson /Robin C. Allshire, Howard J. Cooke. /George Spowart /Fiona M. Ross /Ann C. Chandley, Robert M. Speed, Kun Ma /Adrian T. Sumner, Andrew R. Ross, Elizabeth Graham /Judy Fletcher /Adrian T. Sumner /Adrian T. Sumner /Dieter Schweizer, Peter F. Ambros /Adrian T. Sumner, Arthur R. Mitchell /Joaquina de la Torre, Adrian T. Sumner /Jim Graham, Jim Piper /Nigel P. Carter.

ISBN: 0896032434 0896032892

LCCN: 93034459

Subjects--Topical Terms:
Chromosomes--Research Methodology.
Cytogenetics--Technique.
Human cytogenetics--Technique.

LC Class. No.: QH600 / .C555 1994

Dewey Class. No.: