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Stem cell assays / edited by Mohan C. Vemuri. - xiv, 400 pages : illustrations ; 24 cm. + 1 CD-ROM. - Methods in molecular biology ; 407 . - Methods in molecular biology (Clifton, N.J.) ; 407 .

Includes bibliographical references and index.

Derivation of human embryonic stem cells in xeno-free conditions -- Feeder-layer free culture system for human embryonic stem cells -- Digital imaging of stem cells by electron microscopy -- A controlled-cooling protocol for cryopreservation of human and non-human primate embryonic stem cells -- Cell surface markers in human embryonic stem cells -- Generation of a monoclonal antibody library against human embryonic stem cells -- Analytical methods for cancer stem cells -- Micro RNA profiling an easy and rapid method to screen and characterize stem cell populations -- Gene transfer via nucleofection into adult and embryonic stem cells -- RNAi knockdown of transcription factor Pu.1 in the differentiation of mouse embryonic stem cells -- StemBase a resource for the analysis of stem cell gene expression data -- Isolation of stem cells from human umbilical cord blood -- Ex vivo expansion of hematopoietic stem cells from human cord blood in serum-free conditions -- Hematopoietic colony-forming cell assays -- Assays for alloreactive responses by PCR -- Immune properties of mesenchymal stem cells -- Clinical grade expansion of human bone marrow mesenchymal stem cells -- Adenoviral transduction of mesenchymal stem cells -- Directed differentiation of human embryonic stem cells to dendritic cells -- Insulin-producing cells from embryonic stem cells to dendritic cells -- Insulin-producing cells from embryonic stem cells experimental considerations -- Efficient generation of dopamine neurons from human embryonic stem cells -- Isolation of oligodendroglial cells from cultured neural stem/progenitors -- Differentiation of human embryonic stem cells toward the chondrogenic lineage -- Cartilage tissue engineering directed differentiation of embryonic stem cells in three-dimensional hydrogel culture / Mohan C. Vemuri ... [and others] / Michal Amit / A. Henry Sathananthan and Stefania A. Nottola / Carol B. Ware and Szczepan W. Baran / Raj R. Rao, Alison Venable Johnson, and Steven L. Stice / Micha Drukker, Christina Muscat, and Irving L. Weissman / Vinagolu K. Rajasekhar / Uma Lakshmipathy ... [and others] / Uma Lakshmipathy, Shannon Buckley, and Catherine Verfaillie / Gang-Ming Zou, Meredith A. Thompson, and Mervin C. Yoder / Christopher J. Porter ... [and others] / Nishanth P. Reddy, Mohan C. Vemuri, and Reddanna Pallu / Chao-Ling Yao and Shiaw-Min Hwang / Carla Pereira, Emer Clarke, and Jackie Damen / Patrick Stordeur / Panagiota A. Sotiropoulou and Michael Papamichail / Panagiota A. Sotiropoulou, Sonia A. Perez, and Michael Papamichail / Pablo Bosch and Steven L. Stice / Maxim A. Vodyanik and Igor I. Sluvkin / Maxim A. Vodyanik and Igor I. Slukvin / Enrique Roche ... [and others] / Chang-Hwan Park and Sang-Hun Lee / Frank Zeigler and Stephen G. Hall / Wei Seong Toh ... [and others] / Nathaniel S. Hwang, Shyni Varghese, and Jennifer Elisseeff.

ISBN: 9781588297440 1588297446 9781597455367 1597455369

LCCN: 2007930587

Subjects--Topical Terms:
Stem cells.
Biological assay.
Stem Cells--Laboratory Manuals.
Colony-Forming Units Assay--methods--Laboratory Manuals.

LC Class. No.: QH588.S83 / S73877 2007

Dewey Class. No.:

National Library of Medicine Call No.: QU 325 / S82358 2007