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Stem cells and aging : methods and protocols / edited by Kursad Turksen. - xi, 179 pages : illustrations (some color) ; 27 cm. - Methods in molecular biology, v. 976 1064-3745 ; Springer protocols . - Methods in molecular biology (Clifton, N.J.) ; v. 976. Springer protocols (Series) .

Includes bibliographical references and index.

Comprehensive hematopoietic stem cell isolation methods / Serial transplantation of bone marrow to test self-renewal capacity of hematopoietic stem cells in vivo / Mouse hematopoietic stem cell transplantation / Isolation, characterization, and transplantation of adult liver progenitor cells / Isolation of muscle-derived stem/progenitor cells based on adhesion characteristics to collagen-coated surfaces / Human myoblasts from skeletal muscle biopsies in vitro culture preparations for morphological and cytochemical analyses at light and electron microscopy / Cardiac stem cell senescence / Isolation of mesenchymal stem cells from human bone and long-term cultivation under physiologic oxygen conditions / Methods for assessing effects of Wnt/[beta]-catenin signaling in senescence of mesenchymal stem cells / Intra-femoral injection of human mesenchymal stem cells / Tracking of replicative senescence in mesenchymal stem cells by colony-forming unit frequency / Chromatin immunoprecipitation coupled by quantitative real-time PCR as a tool for analyzing epigenetic regulation of stem cell aging / Quantitative fluorescence in situ hybridization on paraffin embedded tissue / Kyle Rector, Yi Liu, and Gary Van Zant -- Charusheila Ramkumar, Rachel M. Gerstein, and Hong Zhang -- Hui Cheng, Paulina H. Liang, and Tao Cheng -- Mladen I. Yovchev, Mariana D. Dabeva, and Michael Oertel -- Mitra Lavasani ... [and others] -- Manuela Malatesta ... [and others] -- Daniela Cesselli ... [and others] -- Sebastian Klepsch ... [and others] -- Hai-jie Wang and Yu-zhen Tan -- Sindhu T. Mohanty and Ilaria Bellantuono -- Anne Schellenberg, Hatim Hemeda, and Wolfgang Wagner -- Seunghee Lee, Ji-Won Jung, and Kyung-Sun Kang -- Mario Ricciardi, Mauro Krampera, and Marco Chilosi.

ISBN: 9781627033169 1627033165

LCCN: 2013931174

Subjects--Topical Terms:
Stem cells--Laboratory manuals.
Aging--Molecular aspects--Laboratory manuals.
Stem Cells--cytology--Laboratory Manuals.
Aging--physiology--Laboratory Manuals.

LC Class. No.: QH588.S83 / S74297 2013

National Library of Medicine Call No.: QU 375 / S74297 2013