Practical HPLC method development / Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch.

"A Wiley-Interscience publication."

Includes bibliographical references and index.

1. Getting Started -- 2. Basics of Separation -- 3. Detection Sensitivity and Selectivity -- 4. Sample Preparation -- 5. Column -- 6. Non-ionic Samples: Reversed- and Normal-Phase HPLC -- 7. Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC -- 8. Gradient Elution -- 9. Systematic Approach to the Reversed-Phase Separation of Regular Samples -- 10. Computer-Assisted Method Development -- 11. Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds -- 12. Chiral Separations -- 13. Preparative HPLC Separation -- 14. Quantitation (Including Trace Analysis) -- 15. Completing the Method: Validation and Transfer -- App. I. Plate Number and Resolution -- App. II. Properties of Solvents Used in HPLC -- App. III. Retention in Reversed-Phase and Normal-Phase HPLC as a Function of Sample Molecular Structure -- App. IV. Preparing Buffered Mobile Phases -- App. V. Characterizing the Differences Among C[subscript 8] or C[subscript 18] Reversed-Phase Columns from Different Suppliers -- App. VI. Adjusting Mobile-Phase Water Content for Normal-Phase HPLC.

Thoroughly revised and greatly expanded, the Second Edition of this landmark work brings scientists completely up to date on method development for HPLC, one of the main analytical techniques in use today. The authors, widely recognized as preeminent authorities in the field, offer the most far-reaching treatment available, taking into account advances in chromatography and the increasingly complex sample types that have become significant in the past decade.