TY  - BOOK
AU  - Sharon,Amir
TI  - Molecular and cell biology methods for fungi
T2  - Methods in molecular biology,
SN  - 9781607616108
AV  - QK604.2.M64 M645 2010
PY  - 2010///]
CY  - New York
PB  - Humana
KW  - Fungi
KW  - cytology
KW  - Laboratory Manuals
KW  - Molecular Biology
KW  - methods
KW  - Fungal molecular biology
KW  - Laboratory manuals
N1  - Includes bibliographical references and index; -- Protoplast transformation of filamentous fungi; / B. Gillian Turgeon ... [and others]; -- Electroporation and Agrobacterium-mediated spore transformation; / Anna Minz and Amir Sharon; -- High-throughput construction of gene deletion cassettes for generation of Neurospora crassa knockout strains; / Patrick D. Collopy ... [and others]; -- Development of impala based transposon systems for gene tagging in filamentous fungi; / Marie Dufresne and Marie-Josée Daboussi; -- DelsGate : a robust and rapid method for gene deletion; / María D. García-Pedrajas ... [and others]; -- Gene silencing for functional analysis : assessing RNAi as a tool for manipulation of gene expression; / Carmit Ziv and Oded Yarden; -- Analysis of fungal gene expression by real time quantitative PCR; / Shahar Ish-Shalom and Amnon Lichter; -- Identification of differentially expressed fungal gene in planta by suppression subraction hybridization; / Benjamin A. Horwitz and Sophie Lev; -- Quantification of fungal infection of leaves with digital images and scion image software; / Paul H. Goodwin and Tom Hsiang; -- Expression profiling of fungal genes during arbuscular mycorrhiza symbiosis establishment using direct fluorescent in situ RT-PCR; / Pascale M.A. Seddas-Dozolme ... [and others]; -- Application of laser microdissection to study plant-fungal pathogen interactions; / John Fosu-Nyarko, Michael G.K. Jones, and Zhaohui Wang; -- Multiplex gene expression analysis by TRAC in fungal cultures; / Jari J. Rautio; -- Amlification of fungal genomes using multiple displacement amplification; / Simon J. Foster and Brendon J. Monahan; -- Biochemical methods used to study the gene expression and protein complexes in the filamentous fungus Neurospora crassa; / Jinhu Guo ... [and others]; -- Measuring protein kinase and sugar kinase activity in plant pathogenic Fusarium species; / Burton H Bluhm and Xinhua Zhao; -- A detailed protocol for chromatin immunoprecipitation in the yeast Saccharomyces cerevisiae; / Melanie Grably and David Engelberg; -- A method to visualize the actin and microtubule cytoskeleton by indirect immunofluorescence; / Flora Banuett; -- Fluorescence in situ hybridization for molecular cytogenetic analysis in filamentous fungi; / Dai Tsuchiya and Masatoki Taga; -- Live-cell imaging of microtubule dynamics in hyphae of Neurospora crassa; / Maho Uchida, Rosa R. Mouriño-Pérez, and Robert W. Roberson; -- Methods to detect apoptotic-like cell death in filamentous fungi; / Camile P. Semighini and Steven D. Harris; -- Evaluation of antifungal susceptibility using flow cytometry; / Cidália Pina-Vaz and Acácio Gonçalves Rodrigues; -- Preparation of fungi for ultrastructural investigations and immunogoldlabelling; / Gerd Hause and Simone Jahn; -- Split-EGFP screens for the detection and localisation of protein-protein interactions in living yeast cells; / Emma Barnard and David J. Timson
ER  -