TY  - BOOK
AU  - Old,R.W.
AU  - Primrose,S.B.
TI  - Principles of gene manipulation: an introduction to genetic engineering 
T2  - Studies in microbiology
SN  - 0632037121
AV  - QH442 .O42 1994
PY  - 1994///
CY  - Oxford, Boston
PB  - Blackwell Scientific
KW  - Genetic engineering
KW  - Recombinant DNA
N1  - Includes bibliographical references (pages 412-464) and index; Preface; Abbreviations and conversion scale; 1. Gene manipulation: an all-embracing technique; 2. Basic techniques; 3. Cutting and joining DNA molecules; 4. Plasmids as cloning vehicles for use in E. coli; 5. Bacteriophage and cosmid vectors for E. coli; 6. Cloning strategies, gene libraries and cDNA cloning; 7. Recombinant selection and screening; 8. Expression in E. coli of cloned DNA molecules; 9. Analysing DNA sequences; 10. The polymerase chain reaction; 11. Changing genes: site-directed mutagenesis; 12. Cloning in bacteria other than E. coli; 13. Cloning in Saccharomyces cerevisiae and other microbial eukaryotes; 14. Gene transfer to plants; 15. Introducing genes into animal cells; 16. Transferring genes into animal oocytes, eggs, embryos and specific animal tissues; 17. The impact of recombinant DNA technology: the generation of novelty; . Appendix 1: Restriction endonuclease target sites; Appendix 2: The genetic code and single-letter amino acid designations; References; Index
ER  -