TY  - BOOK
AU  - Schwartz,Philip H.
AU  - Wesselschmidt,Robin L.
TI  - Human pluripotent stem cells: methods and protocols 
T2  - Methods in molecular biology
SN  - 9781617792007
AV  - QH588.S83 H8638 2011
PY  - 2011///]
CY  - New York
PB  - Humana
KW  - Stem cells
KW  - Pluripotent Stem Cells
N1  - Includes bibliographical references and index; Stem cell laboratory; design, equipment, and oversight; Robin L. Wesselschmidt and Philip H. Schwartz --; Stem cell banks; preserving cell lines, maintaining genetic integrity and advancing research; Lyn Healy, Lesley Young, and Glyn N. Stacey --; Embryonic stem cell derivation from human embryos; Paul Lerou --; Derivation of human parthenogenetic stem cell lines; Nikolay Turovets ... [ and others] --; Generation of induced pluripotent stem cell lines from human fibroblasts via retroviral gene transfer; Justine D. Miller and Thorsten M. Schlaeger --; Derivation of induced pluripotent stem cells by lentiviral transduction; Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz --; Transgene-free production of pluripotent stem cells using piggyBac transposons; Knut Woltjen ... [and others] --; Traditional human embryonic stem cell culture; Philip H. Schwartz ... [ and others] --; Xeno-free culture of human pluripotent stem cells; Rosita Bergstrom ... [ and others] --; Adaptation of human pluripotent stem cells to feeder-free conditions in chemically defined medium with enzymatic single-cell passaging; Alexander E. Stover and Philip H. Schwartz --; GMP scale-up and banking of pluripotent stem cells for cellular therapy applications; Lara J. Ausubel, Patricia M. Lopez, and Larry A. Couture --; Culture of human pluripotent stem cells on glass slides for high-resolution imaging; Victoria Fox --; Classical cytogenetics; karyotyping techniques; Steven E. Bates --; FISH analysis of human pluripotent stem cells; Suzanne E. Peterson, Jerold Chun, and Jeanne Loring --; Immunocytochemical analysis of human pluripotent stem cells; Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz --; Flow cytometric analysis of human pluripotent stem cells; Mirabelle S.H. Ho, Andrew Fryga, and Andrew L. Laslett --; Teratoma assay; an in vivo assessment of pluripotency; Robin L. Wesselschmidt --; Detection of copy number variation using SNP genotyping; Gregory M. Cooper and Heather C. Mefford --; Genome-wide epigenetic analysis of human pluripotent stem cells by ChIP and ChIP-Seq; Michael J. Hitchler and Judd C. Rice --; Basic approaches to gene expression analysis of stem cells by microarrays; Bernhard Schuldt ... [ and others] --; Human pluripotent stem cells; the development of high-content screening strategies; Sean P. Sherman ... [ and others] --; Quantitative proteome and phosphoproteome analysis of human pluripotent stem cells; Javier Munoz and Albert J.R. Heck --; Lentivirus-mediated modification of pluripotent stem cells; Ruchi Bajpai --; Nucleofection of human embryonic stem cells; Helen Fong ... [and others] --; Nonviral gene delivery in neural progenitors derived from human pluripotent stem cells; Sujoy K. Dhara ... [ and others] --; Gene targeting in human pluripotent stem cells; Ying Liu and Mahendra Rao --; Episomal transgene expression in pluripotent stem cells; Michele M.P. Lufino ... [and others] --; Generation of embryoid bodies from feeder-based or feeder-free human pluripotent stem cell cultures; Alexander E. Stover and Philip H. Schwartz --; Derivation of oligodendrocyte progenitor cells from human embryonic stem cells; Jason Sha ... [and others] --; Directed differentiation of dopamine neurons from human pluripotent stem cells; Lixiang Ma, Yan Liu, and Su-Chun Zhang --; Methods for the derivation and use of cardiomyocytes from human pluripotent stem cells; Wei-Zhong Zhu, Benjamin Van Biber, and Michael A. Laflamme --; In vivo evaluation of putative hematopoietic stem cells derived  from human pluripotent stem cells; Melinda K. Hexum, Xinghui Tian, and Dan S. Kaufman --; Differentiation of dendritic cells from human embryonic stem cells; Kathryn M. Silk ... [and others]
ER  -