TY - BOOK AU - Turksen,Kursad TI - Stem cells and aging: methods and protocols T2 - Methods in molecular biology, SN - 9781627033169 AV - QH588.S83 S74297 2013 PY - 2013///] CY - New York PB - Humana Press KW - Stem cells KW - Laboratory manuals KW - Aging KW - Molecular aspects KW - Stem Cells KW - cytology KW - Laboratory Manuals KW - physiology N1 - Includes bibliographical references and index; Comprehensive hematopoietic stem cell isolation methods; Kyle Rector, Yi Liu, and Gary Van Zant --; Serial transplantation of bone marrow to test self-renewal capacity of hematopoietic stem cells in vivo; Charusheila Ramkumar, Rachel M. Gerstein, and Hong Zhang --; Mouse hematopoietic stem cell transplantation; Hui Cheng, Paulina H. Liang, and Tao Cheng --; Isolation, characterization, and transplantation of adult liver progenitor cells; Mladen I. Yovchev, Mariana D. Dabeva, and Michael Oertel --; Isolation of muscle-derived stem/progenitor cells based on adhesion characteristics to collagen-coated surfaces; Mitra Lavasani ... [and others] --; Human myoblasts from skeletal muscle biopsies; in vitro culture preparations for morphological and cytochemical analyses at light and electron microscopy; Manuela Malatesta ... [and others] --; Cardiac stem cell senescence; Daniela Cesselli ... [and others] --; Isolation of mesenchymal stem cells from human bone and long-term cultivation under physiologic oxygen conditions; Sebastian Klepsch ... [and others] --; Methods for assessing effects of Wnt/[beta]-catenin signaling in senescence of mesenchymal stem cells; Hai-jie Wang and Yu-zhen Tan --; Intra-femoral injection of human mesenchymal stem cells; Sindhu T. Mohanty and Ilaria Bellantuono --; Tracking of replicative senescence in mesenchymal stem cells by colony-forming unit frequency; Anne Schellenberg, Hatim Hemeda, and Wolfgang Wagner --; Chromatin immunoprecipitation coupled by quantitative real-time PCR as a tool for analyzing epigenetic regulation of stem cell aging; Seunghee Lee, Ji-Won Jung, and Kyung-Sun Kang --; Quantitative fluorescence in situ hybridization on paraffin embedded tissue; Mario Ricciardi, Mauro Krampera, and Marco Chilosi ER -