TY - BOOK AU - Primrose,S.B. AU - Twyman,Richard M. AU - Primrose,S.B. AU - Primrose,S.B. TI - Principles of gene manipulation and genomics SN - 1405135441 AV - QH442 .O42 2006 PY - 2006/// CY - Malden, MA, Oxford PB - Blackwell Pub. KW - Genetic engineering KW - Genomics KW - Gene mapping KW - Nucleotide sequence KW - Genetic Engineering KW - Base Sequence KW - Chromosome Mapping KW - DNA, Recombinant N1 - Rev. edition of: Principles of gene manipulation. 6th edition 2001 and Principles of genome analysis and genomics; Includes bibliographical references (pages [547]-626) and index; -- Gene manipulation in the post-genomics era; -- Basic techniques; -- Cutting and joining DNA molecules; -- Basic biology of plasmid and phage vectors; -- Cosmids, phasmids, and other advanced vectors; -- Gene cloning strategies; -- Sequencing genes and short stretches of DNA; -- Changing genes : site-directed mutagenesis and protein engineering; -- Bioinformatics; -- Cloning in bacteria other than Escherichia coli; -- Cloning in Saccharomyces cerevisiaea and other fungi; -- Gene transfer to animal cells; -- Genetic manipulation of animals; -- Gene transfer to plants; -- Advanced transgenic technology; -- The organization and structure of genomes; -- Mapping and sequencing genomes; -- Comparative genomics; -- Large-scale mutagenesis and interference; -- Analysis of the transcriptome; -- Proteomics I : expression analysis and characterization of proteins; -- Proteomics II : analysis of protein structures; -- Proteomics III : protein interactions; -- Metabolomics and global biochemical networks; -- Applications of genomics : understanding the basis of polygenic disorders and identifying quantitative trait loci; -- Applications of recombinant DNA technology ER -