TY  - BOOK
AU  - Boyer,Rodney F
TI  - Modern experimental biochemistry
T2  - Benjamin/Cummings series in the life sciences and chemistry
SN  - 0805331115
AV  - QP519.7 .B68 2000
PY  - 2000///]
CY  - San Francisco
PB  - Benjamin Cummings
KW  - Biochemistry
KW  - Methodology
KW  - Laboratory manuals
KW  - Molecular biology
KW  - methods
KW  - Microbiological Techniques
KW  - Molecular Biology
N1  - Includes bibliographical references and index; part I. Theory and Experimental Techniques; -- Ch. 1. Introduction to the Biochemistry Laboratory; -- A. Safety in the Laboratory; -- B. The Laboratory Notebook; -- C. Cleaning Laboratory Glassware; -- D. Quantitative Transfer of Liquids; -- E. Preparation and Storage of Solutions; -- F. Statistical Analysis of Experimental Measurements; -- Ch. 2. General Laboratory Procedures; -- A. pH Measurements, Buffers, and Electrodes; -- B. Dialysis and Ultrafiltration; -- C. Lyophilization (Freeze-Drying); -- D. Measurement of Protein Solutions; -- E. Measurement of Nucleic Acid Solutions by Spectrophotometry; -- Ch. 3. Separation and Purification of Biomolecules by Chromatography; -- A. Introduction to Chromatography; -- B. Paper and Thin-Layer Chromatography; -- C. Gas Chromatography; -- D. Column Chromatography (GC); -- E. Ion-Exchange Chromatography; -- F. Gel Exclusion Chromatography; -- G. High-Performance Liquid Chromatography (HPLC); -- H. Affinity Chromatography and Immunoadsorption; -- I. Perfusion Chromatography; -- Ch. 4. Characterization of Proteins and Nucleic Acids by Electrophoresis; -- A. Theory of Electrophoresis; -- B. Methods of Electrophoresis; -- C. Practical Aspects of Electrophoresis; -- Ch. 5. Spectroscopic Analysis of Biomolecules; -- A. Ultraviolet-Visible Absorption Spectrophotometry; -- B. Fluorescence Spectrophotometry; -- Ch. 6. Radioisotopes in Biochemical Research; -- A. Origin and Properties of Radioactivity; -- B. Detection and Measurement of Radioactivity; -- C. Safety Rules for Handling Radioactive Materials; -- Ch. 7. Centrifugation of Biomolecules; -- A. Basic Principles of Centrifugation; -- B. Instrumentation for Centrifugation; -- C. Applications of Centrifugation; -- part II. Experiments; -- Experiment 1 Using the Biochemical Literature; -- Experiment 2 Analytical Methods for Amino Acid Separation and Identification; -- Experiment 3 Purification and Characterization of [alpha]-Lactalbumin, a Milk Protein; -- Experiment 4 Structural Analysis of a Dipeptide or Tripeptide; -- Experiment 5 Protein-Ligand Interactions: Binding of Coomassie Blue Dye to Ovalbumin; -- Experiment 6 Kinetic Analysis of an Enzyme, Tyrosinase; -- Experiment 7 Purification and Characterization of a Lipid Extracted from Nutmeg; -- Experiment 8 Fatty Acid Content of Triacylglycerols in Natural Oils; -- Experiment 9 Identification of Carbohydrates by Polarimetry; -- Experiment 10 Characterization of Hemoglobin A[subscript 1c] by Affinity Chromatography; -- Experiment 11 Isolation, Separation and Spectrophotometric Characterization of Photosynthetic Pigments; -- Experiment 12 Photoinduced Proton Transport Through Chloroplast Membranes; -- Experiment 13 Isolation, Subfractionation and Enzymatic Analysis of Beef Heart Mitochondria; -- Experiment 14 Cholesterol (Total and HDL) and Uric Acid Content of Blood Serum: Enzymes as Reagents in Clinical Chemistry; -- Experiment 15 Vitamin C Content of Fruits, Vegetables and Other Foods; -- Experiment 16 Activity and Thermal Stability of Acrylamide Gel-Immobilized Peroxidase: An Experiment in Biotechnology; -- Experiment 17 Extraction of Chromosomal DNA from Bacterial Cells; -- Experiment 18 Characterization of Nucleic Acids by Fluorescence of Bound Ethidium Bromide; -- Experiment 19 Preparation, Isolation, and Purification of Bacterial Plasmid DNA; -- Experiment 20 Rapid, Microscale Isolation of Plasmid DNA; -- Experiment 21 The Action of Restriction Endonucleases on Plasmid or Viral DNA; -- Experiment 22 Specific Cleavage of an Oligomeric Ribonucleotide Substrate by a Ribozyme (RNA Enzyme); -- App. I Properties of Common Acids and Bases; -- App. II Properties of Common Buffer Compounds; -- App. III pK[subscript a] Values and pH[subscript l] Values of Amino Acids; -- App. IV Molecular Weights of Some Common Proteins; -- App. V Common Abbreviations used in this Text; -- App. VI Units of Measurement; -- App. VII Table of the Elements; -- App. VIII Values of t for Analysis of Statistical Confidence Limits; -- App. IX Solutions to Selected Questions
ER  -