TY  - BOOK
AU  - Sambrook,Joseph
AU  - Russell,David W.
TI  - Molecular cloning: a laboratory manual 
SN  - 0879695773
AV  - QH442.2 .S26 2001
PY  - 2001///]
CY  - Cold Spring Harbor, N.Y.
PB  - Cold Spring Harbor Laboratory Press
KW  - Molecular cloning
KW  - Laboratory manuals
KW  - Cloning, Molecular
KW  - Laboratory Manuals
KW  - Clonamiento molecular
KW  - Manuales de laboratorio
N1  - Includes bibliographical references and index; Volume 1: Ch. 1. Plasmids and their usefulness in molecular cloning; -- Ch. 2. Bacteriophage and its vectors; -- Ch. 3. Working with bacteriophage M13 vectors; -- Ch. 4. Working with high-capacity vectors; -- Ch. 5. Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis; -- Ch. 6. Preparation and analysis of eukaryotic genomic DNA; -- Ch. 7. Extraction, purification, and analysis of mRNA from eukaryotic cells; -- Vol. 2: Ch. 8. In Vitro amplification of DNA by the polymerase chain reaction; -- Ch. 9. Preparation of radiolabeled DNA and RNA probes; -- Ch. 10. Working with synthetic oligonucleotide probes; -- Ch. 11. Preparation of cDNA libraries and gene identification; -- Ch. 12. DNA sequencing; -- Ch. 13. Mutagenesis; -- Ch. 14. Screening expression libraries; -- Vol. 3: Ch. 15. Expression of cloned genes in Escherichia coli; -- Ch. 16. Introducing cloned genes into cultured mammalian cells; -- Ch. 17. Analysis of gene expression in cultured mammalian cells; -- Ch. 18. Protein interaction technologies
N2  - DSU Title III 2002-2012
ER  -