Principles of gene manipulation : an introduction to genetic engineering / R.W. Old, S.B. Primrose.

Includes bibliographical references (pages 412-464) and index.

Preface . Abbreviations and conversion scale 1. Gene manipulation: an all-embracing technique 2. Basic techniques 3. Cutting and joining DNA molecules 4. Plasmids as cloning vehicles for use in E. coli 5. Bacteriophage and cosmid vectors for E. coli 6. Cloning strategies, gene libraries and cDNA cloning 7. Recombinant selection and screening 8. Expression in E. coli of cloned DNA molecules 9. Analysing DNA sequences 10. The polymerase chain reaction 11. Changing genes: site-directed mutagenesis 12. Cloning in bacteria other than E. coli 13. Cloning in Saccharomyces cerevisiae and other microbial eukaryotes 14. Gene transfer to plants 15. Introducing genes into animal cells 16. Transferring genes into animal oocytes, eggs, embryos and specific animal tissues 17. The impact of recombinant DNA technology: the generation of novelty . Appendix 1: Restriction endonuclease target sites Appendix 2: The genetic code and single-letter amino acid designations References Index