Analytical biochemistry / David J. Holme and Hazel Peck.

Includes bibliographical references and index.

General principles of analytical biochemistry -- Selection of a valid method of analysis -- Quality of data -- Presentation of data -- Spectroscopy -- Interaction of radiation with matter -- Molecular absorptiometry -- Absorptiometer design -- Molecular fluorescence techniques -- Atomic spectroscopy techniques -- Magnetic resonance spectroscopy -- Separation methods -- Principles of separation techniques -- Methods based on polarity -- Methods based on ionic nature -- Methods based on size -- Methods based on shape -- Electroanalytical methods -- Potentiometry -- Conductimetry -- Coulometry -- Voltammetry -- Biosensors -- Radio-isotopes -- Nature of radioactivity -- Detection and measurement of radioactivity -- Biochemical uses of isotopes -- Automated methods of analysis -- Discrete analysers -- Flow analysis -- Robotics -- Immunological methods -- General processes of the immune response -- Antigen-antibody reactions -- Analytical techniques-precipitation reactions -- Analytical techniques-immunoassay -- Enzymes -- Nature of enzymes -- Monitoring techniques -- Enzyme assay methods.

(cont) Substrate assay methods -- Immobilized enzymes -- Treatment of cells and tissues for enzyme assay -- Carbohydrates -- General structure and function -- Chemical methods of carbohydrate analysis -- Enzymic methods of carbohydrate analysis -- Separation and identification of carbohydrate mixtures -- Amino acids -- General structure and properties -- General reactions -- N-terminal analysis -- Reactions of specific amino acids -- Separation of amino acid mixtures -- Amino acid analyser -- Proteins -- Protein structure -- General methods of quantitation -- Separation of proteins -- Lipids -- Fatty acids -- Simple lipids -- Complex lipids -- Lipid-protein structures -- Sample preparation and handling -- Quantitative methods -- Separation of lipid mixtures -- Nucleic acids -- Nucleic acid composition and structure -- Isolation and purification of nucleic acids -- Methods of nucleic acid analysis -- Vectors -- DNA sequencing -- Numbering and classification of enzymes.