000			04334nam a2200397 i 4500
008			100201s2010 nyua b 001 0 eng
010			_a2010923232
020			_a9781607616108 _qhbk. : alk. paper
020			_a1607616106 _qhbk. : alk. paper
020			_a9781607616115 _qe-ISBN
020			_a1607616114 _qe-ISBN
040			_aNLM _beng _cNLM _dUKM _dBTCTA _dYDXCP _dOMM _dCUS _dC#P _dAGL _dBWX _dCOU _dGEBAY _dSBM _dBAUN _erda
049			_aBAUN_MERKEZ
050	0	4	_aQK604.2.M64 _bM645 2010
082	0	4	_222
245	0	0	_aMolecular and cell biology methods for fungi / _cedited by Amir Sharon
264		1	_aNew York : _bHumana, _c[2010]
264		4	_c©2010
300			_axi, 321 pages : _billustrations ; _c27 cm
336			_atext _btxt _2rdacontent
337			_aunmediated _bn _2rdamedia
338			_avolume _bnc _2rdacarrier
490	1		_aMethods in molecular biology, _x1064-3745 ; _v638
490	1		_aSpringer protocols
504			_aIncludes bibliographical references and index
505	0	0	_t-- Protoplast transformation of filamentous fungi _r/ B. Gillian Turgeon ... [and others] _t-- Electroporation and Agrobacterium-mediated spore transformation _r/ Anna Minz and Amir Sharon _t-- High-throughput construction of gene deletion cassettes for generation of Neurospora crassa knockout strains _r/ Patrick D. Collopy ... [and others] _t-- Development of impala based transposon systems for gene tagging in filamentous fungi _r/ Marie Dufresne and Marie-Josée Daboussi _t-- DelsGate : a robust and rapid method for gene deletion _r/ María D. García-Pedrajas ... [and others] _t-- Gene silencing for functional analysis : assessing RNAi as a tool for manipulation of gene expression _r/ Carmit Ziv and Oded Yarden _t-- Analysis of fungal gene expression by real time quantitative PCR _r/ Shahar Ish-Shalom and Amnon Lichter _t-- Identification of differentially expressed fungal gene in planta by suppression subraction hybridization _r/ Benjamin A. Horwitz and Sophie Lev _t-- Quantification of fungal infection of leaves with digital images and scion image software _r/ Paul H. Goodwin and Tom Hsiang _t-- Expression profiling of fungal genes during arbuscular mycorrhiza symbiosis establishment using direct fluorescent in situ RT-PCR _r/ Pascale M.A. Seddas-Dozolme ... [and others] _t-- Application of laser microdissection to study plant-fungal pathogen interactions _r/ John Fosu-Nyarko, Michael G.K. Jones, and Zhaohui Wang _t-- Multiplex gene expression analysis by TRAC in fungal cultures _r/ Jari J. Rautio _t-- Amlification of fungal genomes using multiple displacement amplification _r/ Simon J. Foster and Brendon J. Monahan _t-- Biochemical methods used to study the gene expression and protein complexes in the filamentous fungus Neurospora crassa _r/ Jinhu Guo ... [and others] _t-- Measuring protein kinase and sugar kinase activity in plant pathogenic Fusarium species _r/ Burton H Bluhm and Xinhua Zhao _t-- A detailed protocol for chromatin immunoprecipitation in the yeast Saccharomyces cerevisiae _r/ Melanie Grably and David Engelberg _t-- A method to visualize the actin and microtubule cytoskeleton by indirect immunofluorescence _r/ Flora Banuett _t-- Fluorescence in situ hybridization for molecular cytogenetic analysis in filamentous fungi _r/ Dai Tsuchiya and Masatoki Taga _t-- Live-cell imaging of microtubule dynamics in hyphae of Neurospora crassa _r/ Maho Uchida, Rosa R. Mouriño-Pérez, and Robert W. Roberson _t-- Methods to detect apoptotic-like cell death in filamentous fungi _r/ Camile P. Semighini and Steven D. Harris _t-- Evaluation of antifungal susceptibility using flow cytometry _r/ Cidália Pina-Vaz and Acácio Gonçalves Rodrigues _t-- Preparation of fungi for ultrastructural investigations and immunogoldlabelling _r/ Gerd Hause and Simone Jahn _t-- Split-EGFP screens for the detection and localisation of protein-protein interactions in living yeast cells _r/ Emma Barnard and David J. Timson
650	1	2	_aFungi _xcytology _vLaboratory Manuals
650	2	2	_aMolecular Biology _xmethods _vLaboratory Manuals
650		0	_aFungal molecular biology _vLaboratory manuals
700	1		_aSharon, Amir
830		0	_942803 _aMethods in molecular biology (Clifton, N.J.) ; _vv. 638. _x1064-3745
830		0	_964760 _aSpringer protocols (Series)
900			_a31168
900			_bsatın
942			_2lcc _cKT
999			_c27791 _d27791