000			02106cam a2200337 i 4500
008			940113s1994 enka b 001 0 eng c
010			_a 94000932
020			_a0632037121
035			_a(OCoLC)
040			_aDNLM/DLC _beng _cDLC _dDLC _dBAUN _erda
049			_aBAUN_MERKEZ
050	0	4	_aQH442 _b.O42 1994
082	0	0	_220
100	1		_aOld, R. W. _992317 _eaut
245	1	0	_aPrinciples of gene manipulation : _ban introduction to genetic engineering / _cR.W. Old, S.B. Primrose.
250			_a5th ed.
264		1	_aOxford : _aBoston : _bBlackwell Scientific, _c1994.
300			_avi, 474 pages : _billustrations ; _c25 cm.
336			_2rdacontent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_2rdacarrier _avolume _bnc
490	1		_aStudies in microbiology
504			_aIncludes bibliographical references (pages 412-464) and index.
505	0	0	_tPreface . _tAbbreviations and conversion scale _t1. Gene manipulation: an all-embracing technique _t2. Basic techniques _t3. Cutting and joining DNA molecules _t4. Plasmids as cloning vehicles for use in E. coli _t5. Bacteriophage and cosmid vectors for E. coli _t6. Cloning strategies, gene libraries and cDNA cloning _t7. Recombinant selection and screening _t8. Expression in E. coli of cloned DNA molecules _t9. Analysing DNA sequences _t10. The polymerase chain reaction _t11. Changing genes: site-directed mutagenesis _t12. Cloning in bacteria other than E. coli _t13. Cloning in Saccharomyces cerevisiae and other microbial eukaryotes _t14. Gene transfer to plants _t15. Introducing genes into animal cells _t16. Transferring genes into animal oocytes, eggs, embryos and specific animal tissues _t17. The impact of recombinant DNA technology: the generation of novelty _t. Appendix 1: Restriction endonuclease target sites _tAppendix 2: The genetic code and single-letter amino acid designations _tReferences _tIndex
650		0	_aGenetic engineering _948209
650		0	_aRecombinant DNA _970537
700	1		_aPrimrose, S. B. _994079 _eaut
830		0	_9104293 _aStudies in microbiology.
900			_a3863
942			_2lcc _cKT
999			_c3755 _d3755