000			05166 am a2200397 i 4500
001			42810
008			110627s2011 nyua b 001 0 eng d
010			_a2011932985
020			_a9781617792007 _qalk. paper
020			_a1617792004 _qalk. paper
020			_a9781617792014 _qe-ISBN
035			_a(OCoLC)
040			_aYDXCP _cYDXCP _dRML _dCUS _dUKMGB _dDLC _dBAUN _erda _beng
049			_aBAUN_MERKEZ
050	0	0	_aQH588.S83 _bH8638 2011
060		4	_aQU 325 _bH9183 2011
245	0	0	_aHuman pluripotent stem cells : _bmethods and protocols / _cedited by Philip H. Schwartz, Robin L. Wesselschmidt.
264		1	_aNew York : _bHumana ; _c[2011]
264		1	_bSpringer, _c[2011]
264		4	_c©2011
300			_axix, 468 pages : _billustrations (some color) ; _c26 cm.
336			_2rdacontent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_2rdacarrier _avolume _bnc
490	1		_aMethods in molecular biology ; _vv. 767
490	1		_aSpringer protocols
504			_aIncludes bibliographical references and index.
505	0	0	_tStem cell laboratory _tdesign, equipment, and oversight / _rRobin L. Wesselschmidt and Philip H. Schwartz -- _tStem cell banks _tpreserving cell lines, maintaining genetic integrity and advancing research / _rLyn Healy, Lesley Young, and Glyn N. Stacey -- _tEmbryonic stem cell derivation from human embryos / _rPaul Lerou -- _tDerivation of human parthenogenetic stem cell lines / _rNikolay Turovets ... [ and others] -- _tGeneration of induced pluripotent stem cell lines from human fibroblasts via retroviral gene transfer / _rJustine D. Miller and Thorsten M. Schlaeger -- _tDerivation of induced pluripotent stem cells by lentiviral transduction / _rHubert E. Nethercott, David J. Brick, and Philip H. Schwartz -- _tTransgene-free production of pluripotent stem cells using piggyBac transposons / _rKnut Woltjen ... [and others] -- _tTraditional human embryonic stem cell culture / _rPhilip H. Schwartz ... [ and others] -- _tXeno-free culture of human pluripotent stem cells / _rRosita Bergstrom ... [ and others] -- _tAdaptation of human pluripotent stem cells to feeder-free conditions in chemically defined medium with enzymatic single-cell passaging / _rAlexander E. Stover and Philip H. Schwartz -- _tGMP scale-up and banking of pluripotent stem cells for cellular therapy applications / _rLara J. Ausubel, Patricia M. Lopez, and Larry A. Couture -- _tCulture of human pluripotent stem cells on glass slides for high-resolution imaging / _rVictoria Fox -- _tClassical cytogenetics _tkaryotyping techniques / _rSteven E. Bates -- _tFISH analysis of human pluripotent stem cells / _rSuzanne E. Peterson, Jerold Chun, and Jeanne Loring -- _tImmunocytochemical analysis of human pluripotent stem cells / _rHubert E. Nethercott, David J. Brick, and Philip H. Schwartz -- _tFlow cytometric analysis of human pluripotent stem cells / _rMirabelle S.H. Ho, Andrew Fryga, and Andrew L. Laslett -- _tTeratoma assay _tan in vivo assessment of pluripotency / _rRobin L. Wesselschmidt -- _tDetection of copy number variation using SNP genotyping / _rGregory M. Cooper and Heather C. Mefford -- _tGenome-wide epigenetic analysis of human pluripotent stem cells by ChIP and ChIP-Seq / _rMichael J. Hitchler and Judd C. Rice -- _tBasic approaches to gene expression analysis of stem cells by microarrays / _rBernhard Schuldt ... [ and others] -- _tHuman pluripotent stem cells _tthe development of high-content screening strategies / _rSean P. Sherman ... [ and others] -- _tQuantitative proteome and phosphoproteome analysis of human pluripotent stem cells / _rJavier Munoz and Albert J.R. Heck -- _tLentivirus-mediated modification of pluripotent stem cells / _rRuchi Bajpai -- _tNucleofection of human embryonic stem cells / _rHelen Fong ... [and others] -- _tNonviral gene delivery in neural progenitors derived from human pluripotent stem cells / _rSujoy K. Dhara ... [ and others] -- _tGene targeting in human pluripotent stem cells / _rYing Liu and Mahendra Rao -- _tEpisomal transgene expression in pluripotent stem cells / _rMichele M.P. Lufino ... [and others] -- _tGeneration of embryoid bodies from feeder-based or feeder-free human pluripotent stem cell cultures / _rAlexander E. Stover and Philip H. Schwartz -- _tDerivation of oligodendrocyte progenitor cells from human embryonic stem cells / _rJason Sha ... [and others] -- _tDirected differentiation of dopamine neurons from human pluripotent stem cells / _rLixiang Ma, Yan Liu, and Su-Chun Zhang -- _tMethods for the derivation and use of cardiomyocytes from human pluripotent stem cells / _rWei-Zhong Zhu, Benjamin Van Biber, and Michael A. Laflamme -- _tIn vivo evaluation of putative hematopoietic stem cells derived from human pluripotent stem cells / _rMelinda K. Hexum, Xinghui Tian, and Dan S. Kaufman -- _tDifferentiation of dendritic cells from human embryonic stem cells / _rKathryn M. Silk ... [and others].
650		0	_aStem cells.
650		2	_aPluripotent Stem Cells.
700	1		_aSchwartz, Philip H.
700	1		_aWesselschmidt, Robin L.
830		0	_942803 _aMethods in molecular biology (Clifton, N.J.) ; _vv. 767.
830		0	_964760 _aSpringer protocols (Series)
942			_2lcc _cKT
999			_c41331 _d41331