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040			_aDLC _beng _cDLC _dC#P _dUKM _dNLM _dMUQ _dBAKER _dBTCTA _dYDXCP _dSTF _dIG# _dCNCGM _dBDX _dOCLCF _dOCLCQ _dOCLCO _dDEBBG _dOCLCO _dOCL _dOCLCO _dUtOrBLW _dBAUN _erda
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049			_aBAUN_MERKEZ
050	0	0	_aQP519.7 _b.B68 2000
060	1	0	_aQU 4 _bB791 2000
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100	1		_aBoyer, Rodney F _994358 _eaut
245	1	0	_aModern experimental biochemistry / _cRodney Boyer
250			_aThird edition
264		1	_aSan Francisco : _bBenjamin Cummings, _c[2000]
264		4	_c©2000
300			_axviii, 475 pages : _billustrations ; _c24 cm
490	1		_aBenjamin/Cummings series in the life sciences and chemistry
504			_aIncludes bibliographical references and index
505	0	0	_tpart I. Theory and Experimental Techniques _t-- Ch. 1. Introduction to the Biochemistry Laboratory _t-- A. Safety in the Laboratory _t-- B. The Laboratory Notebook _t-- C. Cleaning Laboratory Glassware _t-- D. Quantitative Transfer of Liquids _t-- E. Preparation and Storage of Solutions _t-- F. Statistical Analysis of Experimental Measurements _t-- Ch. 2. General Laboratory Procedures _t-- A. pH Measurements, Buffers, and Electrodes _t-- B. Dialysis and Ultrafiltration _t-- C. Lyophilization (Freeze-Drying) _t-- D. Measurement of Protein Solutions _t-- E. Measurement of Nucleic Acid Solutions by Spectrophotometry _t-- Ch. 3. Separation and Purification of Biomolecules by Chromatography _t-- A. Introduction to Chromatography _t-- B. Paper and Thin-Layer Chromatography _t-- C. Gas Chromatography _t-- D. Column Chromatography (GC) _t-- E. Ion-Exchange Chromatography _t-- F. Gel Exclusion Chromatography _t-- G. High-Performance Liquid Chromatography (HPLC) _t-- H. Affinity Chromatography and Immunoadsorption _t-- I. Perfusion Chromatography _t-- Ch. 4. Characterization of Proteins and Nucleic Acids by Electrophoresis _t-- A. Theory of Electrophoresis _t-- B. Methods of Electrophoresis _t-- C. Practical Aspects of Electrophoresis _t-- Ch. 5. Spectroscopic Analysis of Biomolecules _t-- A. Ultraviolet-Visible Absorption Spectrophotometry _t-- B. Fluorescence Spectrophotometry _t-- Ch. 6. Radioisotopes in Biochemical Research _t-- A. Origin and Properties of Radioactivity _t-- B. Detection and Measurement of Radioactivity _t-- C. Safety Rules for Handling Radioactive Materials _t-- Ch. 7. Centrifugation of Biomolecules _t-- A. Basic Principles of Centrifugation _t-- B. Instrumentation for Centrifugation _t-- C. Applications of Centrifugation _t-- part II. Experiments _t-- Experiment 1 Using the Biochemical Literature _t-- Experiment 2 Analytical Methods for Amino Acid Separation and Identification _t-- Experiment 3 Purification and Characterization of [alpha]-Lactalbumin, a Milk Protein _t-- Experiment 4 Structural Analysis of a Dipeptide or Tripeptide _t-- Experiment 5 Protein-Ligand Interactions: Binding of Coomassie Blue Dye to Ovalbumin _t-- Experiment 6 Kinetic Analysis of an Enzyme, Tyrosinase _t-- Experiment 7 Purification and Characterization of a Lipid Extracted from Nutmeg _t-- Experiment 8 Fatty Acid Content of Triacylglycerols in Natural Oils _t-- Experiment 9 Identification of Carbohydrates by Polarimetry _t-- Experiment 10 Characterization of Hemoglobin A[subscript 1c] by Affinity Chromatography _t-- Experiment 11 Isolation, Separation and Spectrophotometric Characterization of Photosynthetic Pigments _t-- Experiment 12 Photoinduced Proton Transport Through Chloroplast Membranes _t-- Experiment 13 Isolation, Subfractionation and Enzymatic Analysis of Beef Heart Mitochondria _t-- Experiment 14 Cholesterol (Total and HDL) and Uric Acid Content of Blood Serum: Enzymes as Reagents in Clinical Chemistry _t-- Experiment 15 Vitamin C Content of Fruits, Vegetables and Other Foods _t-- Experiment 16 Activity and Thermal Stability of Acrylamide Gel-Immobilized Peroxidase: An Experiment in Biotechnology _t-- Experiment 17 Extraction of Chromosomal DNA from Bacterial Cells _t-- Experiment 18 Characterization of Nucleic Acids by Fluorescence of Bound Ethidium Bromide _t-- Experiment 19 Preparation, Isolation, and Purification of Bacterial Plasmid DNA _t-- Experiment 20 Rapid, Microscale Isolation of Plasmid DNA _t-- Experiment 21 The Action of Restriction Endonucleases on Plasmid or Viral DNA _t-- Experiment 22 Specific Cleavage of an Oligomeric Ribonucleotide Substrate by a Ribozyme (RNA Enzyme) _t-- App. I Properties of Common Acids and Bases _t-- App. II Properties of Common Buffer Compounds _t-- App. III pK[subscript a] Values and pH[subscript l] Values of Amino Acids _t-- App. IV Molecular Weights of Some Common Proteins _t-- App. V Common Abbreviations used in this Text _t-- App. VI Units of Measurement _t-- App. VII Table of the Elements _t-- App. VIII Values of t for Analysis of Statistical Confidence Limits _t-- App. IX Solutions to Selected Questions
650		0	_aBiochemistry _xMethodology _9103568
650		0	_aBiochemistry _vLaboratory manuals
650		0	_aMolecular biology _xMethodology _977223
650		0	_aMolecular biology _vLaboratory manuals
650	1	2	_aBiochemistry _xmethods
650	2	2	_aMicrobiological Techniques _xmethods
650	2	2	_aMolecular Biology _xmethods
830		0	_9110300 _aBenjamin/Cummings series in the life sciences and chemistry.
942			_2lcc _cKT
999			_c6107 _d6107