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Molecular and cell biology methods for fungi / edited by Amir Sharon - xi, 321 pages : illustrations ; 27 cm - Methods in molecular biology, 638 1064-3745 ; Springer protocols . - Methods in molecular biology (Clifton, N.J.) ; v. 638. Springer protocols (Series) .

Includes bibliographical references and index

-- Protoplast transformation of filamentous fungi -- Electroporation and Agrobacterium-mediated spore transformation -- High-throughput construction of gene deletion cassettes for generation of Neurospora crassa knockout strains -- Development of impala based transposon systems for gene tagging in filamentous fungi -- DelsGate : a robust and rapid method for gene deletion -- Gene silencing for functional analysis : assessing RNAi as a tool for manipulation of gene expression -- Analysis of fungal gene expression by real time quantitative PCR -- Identification of differentially expressed fungal gene in planta by suppression subraction hybridization -- Quantification of fungal infection of leaves with digital images and scion image software -- Expression profiling of fungal genes during arbuscular mycorrhiza symbiosis establishment using direct fluorescent in situ RT-PCR -- Application of laser microdissection to study plant-fungal pathogen interactions -- Multiplex gene expression analysis by TRAC in fungal cultures -- Amlification of fungal genomes using multiple displacement amplification -- Biochemical methods used to study the gene expression and protein complexes in the filamentous fungus Neurospora crassa -- Measuring protein kinase and sugar kinase activity in plant pathogenic Fusarium species -- A detailed protocol for chromatin immunoprecipitation in the yeast Saccharomyces cerevisiae -- A method to visualize the actin and microtubule cytoskeleton by indirect immunofluorescence -- Fluorescence in situ hybridization for molecular cytogenetic analysis in filamentous fungi -- Live-cell imaging of microtubule dynamics in hyphae of Neurospora crassa -- Methods to detect apoptotic-like cell death in filamentous fungi -- Evaluation of antifungal susceptibility using flow cytometry -- Preparation of fungi for ultrastructural investigations and immunogoldlabelling -- Split-EGFP screens for the detection and localisation of protein-protein interactions in living yeast cells / B. Gillian Turgeon ... [and others] / Anna Minz and Amir Sharon / Patrick D. Collopy ... [and others] / Marie Dufresne and Marie-Josée Daboussi / María D. García-Pedrajas ... [and others] / Carmit Ziv and Oded Yarden / Shahar Ish-Shalom and Amnon Lichter / Benjamin A. Horwitz and Sophie Lev / Paul H. Goodwin and Tom Hsiang / Pascale M.A. Seddas-Dozolme ... [and others] / John Fosu-Nyarko, Michael G.K. Jones, and Zhaohui Wang / Jari J. Rautio / Simon J. Foster and Brendon J. Monahan / Jinhu Guo ... [and others] / Burton H Bluhm and Xinhua Zhao / Melanie Grably and David Engelberg / Flora Banuett / Dai Tsuchiya and Masatoki Taga / Maho Uchida, Rosa R. Mouriño-Pérez, and Robert W. Roberson / Camile P. Semighini and Steven D. Harris / Cidália Pina-Vaz and Acácio Gonçalves Rodrigues / Gerd Hause and Simone Jahn / Emma Barnard and David J. Timson

ISBN: 9781607616108 1607616106 9781607616115 1607616114

LCCN: 2010923232

Subjects--Topical Terms:
Fungi--cytology--Laboratory Manuals
Molecular Biology--methods--Laboratory Manuals
Fungal molecular biology--Laboratory manuals

LC Class. No.: QK604.2.M64 / M645 2010

Dewey Class. No.: