Molecular and cell biology methods for fungi / edited by Amir Sharon

Includes bibliographical references and index

-- Protoplast transformation of filamentous fungi / B. Gillian Turgeon ... [and others] -- Electroporation and Agrobacterium-mediated spore transformation / Anna Minz and Amir Sharon -- High-throughput construction of gene deletion cassettes for generation of Neurospora crassa knockout strains / Patrick D. Collopy ... [and others] -- Development of impala based transposon systems for gene tagging in filamentous fungi / Marie Dufresne and Marie-Josée Daboussi -- DelsGate : a robust and rapid method for gene deletion / María D. García-Pedrajas ... [and others] -- Gene silencing for functional analysis : assessing RNAi as a tool for manipulation of gene expression / Carmit Ziv and Oded Yarden -- Analysis of fungal gene expression by real time quantitative PCR / Shahar Ish-Shalom and Amnon Lichter -- Identification of differentially expressed fungal gene in planta by suppression subraction hybridization / Benjamin A. Horwitz and Sophie Lev -- Quantification of fungal infection of leaves with digital images and scion image software / Paul H. Goodwin and Tom Hsiang -- Expression profiling of fungal genes during arbuscular mycorrhiza symbiosis establishment using direct fluorescent in situ RT-PCR / Pascale M.A. Seddas-Dozolme ... [and others] -- Application of laser microdissection to study plant-fungal pathogen interactions / John Fosu-Nyarko, Michael G.K. Jones, and Zhaohui Wang -- Multiplex gene expression analysis by TRAC in fungal cultures / Jari J. Rautio -- Amlification of fungal genomes using multiple displacement amplification / Simon J. Foster and Brendon J. Monahan -- Biochemical methods used to study the gene expression and protein complexes in the filamentous fungus Neurospora crassa / Jinhu Guo ... [and others] -- Measuring protein kinase and sugar kinase activity in plant pathogenic Fusarium species / Burton H Bluhm and Xinhua Zhao -- A detailed protocol for chromatin immunoprecipitation in the yeast Saccharomyces cerevisiae / Melanie Grably and David Engelberg -- A method to visualize the actin and microtubule cytoskeleton by indirect immunofluorescence / Flora Banuett -- Fluorescence in situ hybridization for molecular cytogenetic analysis in filamentous fungi / Dai Tsuchiya and Masatoki Taga -- Live-cell imaging of microtubule dynamics in hyphae of Neurospora crassa / Maho Uchida, Rosa R. Mouriño-Pérez, and Robert W. Roberson -- Methods to detect apoptotic-like cell death in filamentous fungi / Camile P. Semighini and Steven D. Harris -- Evaluation of antifungal susceptibility using flow cytometry / Cidália Pina-Vaz and Acácio Gonçalves Rodrigues -- Preparation of fungi for ultrastructural investigations and immunogoldlabelling / Gerd Hause and Simone Jahn -- Split-EGFP screens for the detection and localisation of protein-protein interactions in living yeast cells / Emma Barnard and David J. Timson