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Human pluripotent stem cells : methods and protocols / edited by Philip H. Schwartz, Robin L. Wesselschmidt. - xix, 468 pages : illustrations (some color) ; 26 cm. - Methods in molecular biology ; v. 767 Springer protocols . - Methods in molecular biology (Clifton, N.J.) ; v. 767. Springer protocols (Series) .

Includes bibliographical references and index.

Stem cell laboratory design, equipment, and oversight / Stem cell banks preserving cell lines, maintaining genetic integrity and advancing research / Embryonic stem cell derivation from human embryos / Derivation of human parthenogenetic stem cell lines / Generation of induced pluripotent stem cell lines from human fibroblasts via retroviral gene transfer / Derivation of induced pluripotent stem cells by lentiviral transduction / Transgene-free production of pluripotent stem cells using piggyBac transposons / Traditional human embryonic stem cell culture / Xeno-free culture of human pluripotent stem cells / Adaptation of human pluripotent stem cells to feeder-free conditions in chemically defined medium with enzymatic single-cell passaging / GMP scale-up and banking of pluripotent stem cells for cellular therapy applications / Culture of human pluripotent stem cells on glass slides for high-resolution imaging / Classical cytogenetics karyotyping techniques / FISH analysis of human pluripotent stem cells / Immunocytochemical analysis of human pluripotent stem cells / Flow cytometric analysis of human pluripotent stem cells / Teratoma assay an in vivo assessment of pluripotency / Detection of copy number variation using SNP genotyping / Genome-wide epigenetic analysis of human pluripotent stem cells by ChIP and ChIP-Seq / Basic approaches to gene expression analysis of stem cells by microarrays / Human pluripotent stem cells the development of high-content screening strategies / Quantitative proteome and phosphoproteome analysis of human pluripotent stem cells / Lentivirus-mediated modification of pluripotent stem cells / Nucleofection of human embryonic stem cells / Nonviral gene delivery in neural progenitors derived from human pluripotent stem cells / Gene targeting in human pluripotent stem cells / Episomal transgene expression in pluripotent stem cells / Generation of embryoid bodies from feeder-based or feeder-free human pluripotent stem cell cultures / Derivation of oligodendrocyte progenitor cells from human embryonic stem cells / Directed differentiation of dopamine neurons from human pluripotent stem cells / Methods for the derivation and use of cardiomyocytes from human pluripotent stem cells / In vivo evaluation of putative hematopoietic stem cells derived from human pluripotent stem cells / Differentiation of dendritic cells from human embryonic stem cells / Robin L. Wesselschmidt and Philip H. Schwartz -- Lyn Healy, Lesley Young, and Glyn N. Stacey -- Paul Lerou -- Nikolay Turovets ... [ and others] -- Justine D. Miller and Thorsten M. Schlaeger -- Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz -- Knut Woltjen ... [and others] -- Philip H. Schwartz ... [ and others] -- Rosita Bergstrom ... [ and others] -- Alexander E. Stover and Philip H. Schwartz -- Lara J. Ausubel, Patricia M. Lopez, and Larry A. Couture -- Victoria Fox -- Steven E. Bates -- Suzanne E. Peterson, Jerold Chun, and Jeanne Loring -- Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz -- Mirabelle S.H. Ho, Andrew Fryga, and Andrew L. Laslett -- Robin L. Wesselschmidt -- Gregory M. Cooper and Heather C. Mefford -- Michael J. Hitchler and Judd C. Rice -- Bernhard Schuldt ... [ and others] -- Sean P. Sherman ... [ and others] -- Javier Munoz and Albert J.R. Heck -- Ruchi Bajpai -- Helen Fong ... [and others] -- Sujoy K. Dhara ... [ and others] -- Ying Liu and Mahendra Rao -- Michele M.P. Lufino ... [and others] -- Alexander E. Stover and Philip H. Schwartz -- Jason Sha ... [and others] -- Lixiang Ma, Yan Liu, and Su-Chun Zhang -- Wei-Zhong Zhu, Benjamin Van Biber, and Michael A. Laflamme -- Melinda K. Hexum, Xinghui Tian, and Dan S. Kaufman -- Kathryn M. Silk ... [and others].

ISBN: 9781617792007 1617792004 9781617792014

LCCN: 2011932985

Subjects--Topical Terms:
Stem cells.
Pluripotent Stem Cells.

LC Class. No.: QH588.S83 / H8638 2011

National Library of Medicine Call No.: QU 325 / H9183 2011