Human pluripotent stem cells : methods and protocols / edited by Philip H. Schwartz, Robin L. Wesselschmidt.

Includes bibliographical references and index.

Stem cell laboratory design, equipment, and oversight / Robin L. Wesselschmidt and Philip H. Schwartz -- Stem cell banks preserving cell lines, maintaining genetic integrity and advancing research / Lyn Healy, Lesley Young, and Glyn N. Stacey -- Embryonic stem cell derivation from human embryos / Paul Lerou -- Derivation of human parthenogenetic stem cell lines / Nikolay Turovets ... [ and others] -- Generation of induced pluripotent stem cell lines from human fibroblasts via retroviral gene transfer / Justine D. Miller and Thorsten M. Schlaeger -- Derivation of induced pluripotent stem cells by lentiviral transduction / Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz -- Transgene-free production of pluripotent stem cells using piggyBac transposons / Knut Woltjen ... [and others] -- Traditional human embryonic stem cell culture / Philip H. Schwartz ... [ and others] -- Xeno-free culture of human pluripotent stem cells / Rosita Bergstrom ... [ and others] -- Adaptation of human pluripotent stem cells to feeder-free conditions in chemically defined medium with enzymatic single-cell passaging / Alexander E. Stover and Philip H. Schwartz -- GMP scale-up and banking of pluripotent stem cells for cellular therapy applications / Lara J. Ausubel, Patricia M. Lopez, and Larry A. Couture -- Culture of human pluripotent stem cells on glass slides for high-resolution imaging / Victoria Fox -- Classical cytogenetics karyotyping techniques / Steven E. Bates -- FISH analysis of human pluripotent stem cells / Suzanne E. Peterson, Jerold Chun, and Jeanne Loring -- Immunocytochemical analysis of human pluripotent stem cells / Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz -- Flow cytometric analysis of human pluripotent stem cells / Mirabelle S.H. Ho, Andrew Fryga, and Andrew L. Laslett -- Teratoma assay an in vivo assessment of pluripotency / Robin L. Wesselschmidt -- Detection of copy number variation using SNP genotyping / Gregory M. Cooper and Heather C. Mefford -- Genome-wide epigenetic analysis of human pluripotent stem cells by ChIP and ChIP-Seq / Michael J. Hitchler and Judd C. Rice -- Basic approaches to gene expression analysis of stem cells by microarrays / Bernhard Schuldt ... [ and others] -- Human pluripotent stem cells the development of high-content screening strategies / Sean P. Sherman ... [ and others] -- Quantitative proteome and phosphoproteome analysis of human pluripotent stem cells / Javier Munoz and Albert J.R. Heck -- Lentivirus-mediated modification of pluripotent stem cells / Ruchi Bajpai -- Nucleofection of human embryonic stem cells / Helen Fong ... [and others] -- Nonviral gene delivery in neural progenitors derived from human pluripotent stem cells / Sujoy K. Dhara ... [ and others] -- Gene targeting in human pluripotent stem cells / Ying Liu and Mahendra Rao -- Episomal transgene expression in pluripotent stem cells / Michele M.P. Lufino ... [and others] -- Generation of embryoid bodies from feeder-based or feeder-free human pluripotent stem cell cultures / Alexander E. Stover and Philip H. Schwartz -- Derivation of oligodendrocyte progenitor cells from human embryonic stem cells / Jason Sha ... [and others] -- Directed differentiation of dopamine neurons from human pluripotent stem cells / Lixiang Ma, Yan Liu, and Su-Chun Zhang -- Methods for the derivation and use of cardiomyocytes from human pluripotent stem cells / Wei-Zhong Zhu, Benjamin Van Biber, and Michael A. Laflamme -- In vivo evaluation of putative hematopoietic stem cells derived from human pluripotent stem cells / Melinda K. Hexum, Xinghui Tian, and Dan S. Kaufman -- Differentiation of dendritic cells from human embryonic stem cells / Kathryn M. Silk ... [and others].