Modern experimental biochemistry / Rodney Boyer

Includes bibliographical references and index

part I. Theory and Experimental Techniques -- Ch. 1. Introduction to the Biochemistry Laboratory -- A. Safety in the Laboratory -- B. The Laboratory Notebook -- C. Cleaning Laboratory Glassware -- D. Quantitative Transfer of Liquids -- E. Preparation and Storage of Solutions -- F. Statistical Analysis of Experimental Measurements -- Ch. 2. General Laboratory Procedures -- A. pH Measurements, Buffers, and Electrodes -- B. Dialysis and Ultrafiltration -- C. Lyophilization (Freeze-Drying) -- D. Measurement of Protein Solutions -- E. Measurement of Nucleic Acid Solutions by Spectrophotometry -- Ch. 3. Separation and Purification of Biomolecules by Chromatography -- A. Introduction to Chromatography -- B. Paper and Thin-Layer Chromatography -- C. Gas Chromatography -- D. Column Chromatography (GC) -- E. Ion-Exchange Chromatography -- F. Gel Exclusion Chromatography -- G. High-Performance Liquid Chromatography (HPLC) -- H. Affinity Chromatography and Immunoadsorption -- I. Perfusion Chromatography -- Ch. 4. Characterization of Proteins and Nucleic Acids by Electrophoresis -- A. Theory of Electrophoresis -- B. Methods of Electrophoresis -- C. Practical Aspects of Electrophoresis -- Ch. 5. Spectroscopic Analysis of Biomolecules -- A. Ultraviolet-Visible Absorption Spectrophotometry -- B. Fluorescence Spectrophotometry -- Ch. 6. Radioisotopes in Biochemical Research -- A. Origin and Properties of Radioactivity -- B. Detection and Measurement of Radioactivity -- C. Safety Rules for Handling Radioactive Materials -- Ch. 7. Centrifugation of Biomolecules -- A. Basic Principles of Centrifugation -- B. Instrumentation for Centrifugation -- C. Applications of Centrifugation -- part II. Experiments -- Experiment 1 Using the Biochemical Literature -- Experiment 2 Analytical Methods for Amino Acid Separation and Identification -- Experiment 3 Purification and Characterization of [alpha]-Lactalbumin, a Milk Protein -- Experiment 4 Structural Analysis of a Dipeptide or Tripeptide -- Experiment 5 Protein-Ligand Interactions: Binding of Coomassie Blue Dye to Ovalbumin -- Experiment 6 Kinetic Analysis of an Enzyme, Tyrosinase -- Experiment 7 Purification and Characterization of a Lipid Extracted from Nutmeg -- Experiment 8 Fatty Acid Content of Triacylglycerols in Natural Oils -- Experiment 9 Identification of Carbohydrates by Polarimetry -- Experiment 10 Characterization of Hemoglobin A[subscript 1c] by Affinity Chromatography -- Experiment 11 Isolation, Separation and Spectrophotometric Characterization of Photosynthetic Pigments -- Experiment 12 Photoinduced Proton Transport Through Chloroplast Membranes -- Experiment 13 Isolation, Subfractionation and Enzymatic Analysis of Beef Heart Mitochondria -- Experiment 14 Cholesterol (Total and HDL) and Uric Acid Content of Blood Serum: Enzymes as Reagents in Clinical Chemistry -- Experiment 15 Vitamin C Content of Fruits, Vegetables and Other Foods -- Experiment 16 Activity and Thermal Stability of Acrylamide Gel-Immobilized Peroxidase: An Experiment in Biotechnology -- Experiment 17 Extraction of Chromosomal DNA from Bacterial Cells -- Experiment 18 Characterization of Nucleic Acids by Fluorescence of Bound Ethidium Bromide -- Experiment 19 Preparation, Isolation, and Purification of Bacterial Plasmid DNA -- Experiment 20 Rapid, Microscale Isolation of Plasmid DNA -- Experiment 21 The Action of Restriction Endonucleases on Plasmid or Viral DNA -- Experiment 22 Specific Cleavage of an Oligomeric Ribonucleotide Substrate by a Ribozyme (RNA Enzyme) -- App. I Properties of Common Acids and Bases -- App. II Properties of Common Buffer Compounds -- App. III pK[subscript a] Values and pH[subscript l] Values of Amino Acids -- App. IV Molecular Weights of Some Common Proteins -- App. V Common Abbreviations used in this Text -- App. VI Units of Measurement -- App. VII Table of the Elements -- App. VIII Values of t for Analysis of Statistical Confidence Limits -- App. IX Solutions to Selected Questions